|Asst. Prof Navin Kumar Verma |
Development of novel antisense ssDNA-based skin lightening agent
Skin colour is determined by a brown pigment, called melanin, which is produced by melanocytes. The process of melanin synthesis or melanogenesis is catalysed by an enzyme tyrosinase. Therefore, currently used skin lightening formulations contain tyrosinase inhibitors (e.g. hydroquinone, kojic acid) to supress melanogenesis. However, these inhibitors cause several undesirable effects. With better understanding of the melanogenesis pathway, we propose to target a downstream regulator Tyrp1 using antisense ssDNA-based molecules as a safer alternative approach. The aim of this interdisciplinary project is to develop new-generation antisense ssDNA-based molecules specifically targeting Tyrp1 for suppressing melanogenesis in melanocytes that can be used for developing a topical formulation. The student will gain specialized training in cell culture, gene silencing technique, topical drug delivery, microscopy, and variety of molecular and biochemical assays.